inPractice® - Measuring Testosterone Levels for Hypogonadism

In patients with a normal sleep/wake cycle, laboratory assessment of hypogonadism should take place between 8:00 and 10:00 am (Management Guidelines)^{[Testosterone Guidelines]}
In HIV-infected men, especially those with chronic hepatitis, measurement of free testosterone is recommended, either by the equilibrium dialysis method or calculated using the total testosterone measured by liquid chromatography/tandem mass spectrometry, sex hormone binding globulin, and serum albumin^{[Mylonakis 2001; Vermeulen 1999]}
For total testosterone, a morning testosterone < 300 ng/dL is considered hypogonadal^{[Testosterone Guidelines]}