HIV Testing and Diagnosis

  • Author: Peter Leone, MD (More Info)
  • Editors in Chief: Daniel R. Kuritzkes, MD; Joseph J. Eron, Jr., MD
  • Last Reviewed: 1/9/18 (What's New)


  • In high-risk settings or in areas with high-risk populations, HIV testing should be coupled with screening for AHI via the HIV-1 antibody, HIV-2 antibody, and HIV-1 p24 antigen combination immunoassay[CDC HIV Testing; DHHS ART]
    • Reactive specimens should undergo HIV-1/HIV-2 antibody differentiation testing
    • Specimens initially reactive but nonreactive or indeterminate by differentiation testing should be tested for HIV-1 RNA

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